产品名称：马铃薯葡萄糖琼脂
Product name：Potato Dextrose Agar
产品用途：用于霉菌和酵母菌的计数和分离培养。
Product usage: Used for counting and isolating and culturing of mold and yeast.
检验原理：
Test principle:
马铃薯浸粉有助于各种霉菌的生长；葡萄糖提供能源；琼脂是培养基的凝固剂。Potato soaking powder aids in the growth of various molds; glucose provides energy; and agar is a coagulant for the medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
马铃薯浸粉 Potato powder |
6.0 |
|
葡萄糖 Glucose |
20.0 |
|
琼脂 Agar |
20.0 |
|
pH |
5.6±0.2 25℃ |
使用方法
Usage
称取本品46.0g，加入蒸馏水1000ml，搅拌加热煮沸至完全溶解，115℃高压灭菌20分钟，备用。灭菌好的培养基仅可重复加热熔化一次。
注：培养基中使用的马铃薯浸粉中含有较多淀粉类成分，灭菌后的培养基稍浑浊，可能出现少量白色沉淀，摇匀倒平板即可。灭菌前加热煮沸溶解培养基再进行灭菌不易出现沉淀。
Weigh 46.0g of this product, add 1000ml of distilled water, stir and heat to boil until completely dissolved, autoclave at 115℃ for 20 minutes, standby. Sterilized culture medium can only be repeated heating and melting once.
Note: The potato powder used in the medium contains more starch components, the medium is slightly turbid after sterilization, a small amount of white precipitate may appear, shake well and pour the plate can be. Before sterilization, heat and boil to dissolve the culture medium and then sterilize it to make it less prone to precipitation.
