产品名称：LB肉汤
Product name：Bacterial L-growing Broth
产品用途：用于分子生物学中大肠杆菌的培养。
Product usage: Used for the cultivation of e. coli in molecular biology.
检验原理
Test principle
蛋白胨和酵母粉为目标菌生长提供营养；氯化钠维持渗透压平衡。
Peptone and yeast powder provide nutrients for the growth of target bacteria. Sodium chloride maintains osmotic pressure balance.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
胰蛋白胨 Tryptone |
10.0 |
|
酵母浸粉 Yeast infusion powder |
5.0 |
|
氯化钠 Sodium chloride |
10.0 |
|
PH |
7.0±0.2 25℃ |
使用方法
Usage
称取本品25.0克，加入蒸馏水1000mL，搅拌加热煮沸至完全溶解，分装试管或三角瓶，121℃高压灭菌15分钟，备用。
Weigh 25.0 g of this product, add distilled water 1000 mL, stirring heating and boiling until completely dissolved, divided into test tubes or triangular bottles, 121 ℃ autoclave sterilization for 15 minutes, standby.
