产品名称：平板计数琼脂
Product name：PCA
产品用途：用于菌落总数的测定。
Product usage: For the determination of the total number of colonies.
检验原理:
Test principle:
胰蛋白胨提供碳源和氮源；酵母浸粉提供B 族维生素；葡萄糖提供能源；琼脂是培养基的凝固剂。
Tryptone provides carbon and nitrogen sources. Yeast extract powder provides B vitamins; Glucose provides energy; AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
胰蛋白胨 tryptone |
5.0 |
|
酵母浸粉 Yeast infusion powder |
2.5 |
|
葡萄糖 Glucose |
1.0 |
|
琼脂 Agar |
15.0 |
|
pH |
7.0 ± 0.2 25℃ |
使用方法
Usage
称取本品 23.5g，加热搅拌溶解于1000ml 蒸馏水中，分装试管或锥形瓶，121℃高压灭菌15分钟，备用。
Weigh 23.5g of this product, stir and dissolve it in 1000ml distilled water, divide into test tubes or conical bottles, autoclave at 121℃ for 15 minutes, and set aside.
