产品名称:营养肉汤
Product name:Nutrient Broth
产品用途:用于一般细菌培养、转种、复壮、增菌等。
Product usage: Used for general bacterial culture, reseeding, rejuvenation, enrichment, etc.
检验原理
Test principle
蛋白胨和牛肉浸粉提供碳氮源、维生素和生长因子等生长必需的营养物质;氯化钠用于维持培养基的渗透压。
Peptone and beef extract powder provide essential nutrients for growth such as carbon and nitrogen sources, vitamins and growth factors. Sodium chloride is used to maintain osmotic pressure in the medium.
配方成分
Formula ingredients
|
配方 Formula |
含量(g/L) Content (g/L) |
|
蛋白胨 Peptone |
10.0 |
|
牛肉浸粉 Beef infusion powder |
3.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
PH |
7.2±0.2 25℃ |
使用方法
Usage
称取本品18.0g,加入蒸馏水1000ml,搅拌加热煮沸至完全溶解,分装,121℃高压灭菌15分钟,备用。
Weigh 18.0g of this product, add 1000ml of distilled water, stirring heating and boiling until completely dissolved, divided, 121 ℃ autoclave sterilization for 15 minutes, standby.
