产品名称：营养琼脂（NA）
Product name：Nutrient Agar
产品用途：细菌计数、不含糖，可作血琼脂基础和传代用(GB标准)，用于一般细菌培养、转种、复壮和增菌等。
Product usage: Bacteria count, no sugar, can be used as blood agar base and passage (GB standard), for general bacterial culture, transfer, rejuvenation and proliferation.
检验原理：
Test principle:
蛋白胨和牛肉浸粉提供碳氮源、维生素和生长因子等其他营养物质；氯化钠用于维持培养基的渗透压；琼脂为凝固剂。
Peptone and beef infusion powder provide other nutrients such as carbon and nitrogen sources, vitamins and growth factors; Sodium chloride is used to maintain the osmotic pressure of the medium. AGAR is a coagulant.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
10.0 |
|
牛肉膏粉 Beef paste powder |
3.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
琼脂 Agar |
15.0 |
|
pH |
7.3 ± 0.2 25℃ |
使用方法
Usage
称取本品33.0g，加入蒸馏水1000ml，搅拌加热煮沸至完全溶解，121℃高压灭菌15分钟，备用。
Weigh 33.0g of this product, add 1000ml of distilled water, stir and heat to boil until completely dissolved, 121 ℃ autoclave sterilization for 15 minutes, standby.
