产品名称：缓冲蛋白胨水
Product name：BPW
产品用途：用于沙门氏菌前增菌培养，亦可用于李斯特氏菌前增菌培养（GB、SN标准）。
Product usage: Used to increase before salmonella bacteria cultivation, can be used to increase before liszt fungus culture, SN standard (GB).
检验原理
Test Principle
蛋白胨提供碳源和氮源满足细菌生长的需求；氯化钠可维持均衡的渗透压；磷酸二氢钾和磷酸氢二钠是缓冲剂。
Peptone provides carbon source and nitrogen source to meet the needs of bacterial growth; Sodium chloride can maintain a balanced osmotic pressure; Potassium dihydrogen phosphate and disodium hydrogen phosphate are buffers.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
10.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
磷酸氢二钠 Disodium hydrogen phosphate |
3.57 |
|
磷酸二氢钾 Potassium dihydrogen phosphate |
1.5 |
|
PH |
7.2±0.2 25℃ |
使用方法
Usage
称取本品20.1g，加入蒸馏水1000ml，搅拌加热煮沸至完全溶解，121℃高压灭菌15分钟，备用。
Weigh 20.1g of this product, add 1000ml of distilled water, stirring heating and boiling until completely dissolved, 121 ℃ autoclave sterilization for 15 minutes, standby.
