产品名称：脑心浸液肉汤（BHI）
Product name：Brain Heart Infusion Broth
产品用途：营养丰富，用于培养各种细菌。
Product usage: Rich in nutrients, used to culture various bacteria.
检验原理
Test principle
胰蛋白质胨和牛心浸粉提供氮源、维生素和生长因子；葡萄糖可为多种细菌提供能源；氯化钠维持均衡的渗透压；磷酸氢二钠为缓冲剂。
Trypsin peptone and bovine heart infusion powder provide nitrogen sources, vitamins and growth factors; Glucose can provide energy for a variety of bacteria; Sodium chloride maintains a balanced osmotic pressure; Disodium hydrogen phosphate is the buffer.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
胰蛋白胨 Tryptone |
10.0 |
|
牛心浸粉 Beef heart in powder |
17.5 |
|
氯化钠 Sodium chloride |
5.0 |
|
葡萄糖 Glucose |
2.0 |
|
磷酸氢二钠(12H2O) Disodium hydrogen phosphate (12H2O) |
2.5 |
|
pH |
7.4±0.2 25℃ |
使用方法
Usage
称取本品37g，加入1L蒸馏水或去离子水，搅拌加热煮沸至完全溶解，分装试管或三角瓶，121℃灭菌15分钟。
Weigh 37g of this product, add 1L of distilled water or deionized water, stirring heating and boiling until completely dissolved, divided into test tubes or triangular bottles, 121 ℃ sterilized for 15 minutes.
