产品名称：孟加拉红琼脂
Product name：Rose Bengal Agar
产品用途：用于食品中霉菌和酵母菌总数的测定。
Product usage: Used for the determination of the total number of molds and yeasts in food.
检验原理
Test principle
蛋白胨在培养基中作为营养物质提供菌体生长所需氮源等；葡萄糖作为能源；磷酸盐作为缓冲剂；镁盐促进菌体细胞的生长；孟加拉红可抑制细菌的生长，也可限制繁殖快的霉菌菌落的大小和高度，此外还作为着色剂，霉菌或酵母菌吸收后便于菌落的观察；氯霉素为抗生素可抑制细菌的生长；琼脂作为凝固剂。
Peptone is used as a nutrient in the medium to provide nitrogen source for the growth of bacteria. Glucose as an energy source; Phosphate as buffer; Magnesium salts promote the growth of bacterial cells; Bengal red can inhibit the growth of bacteria, can also limit the size and height of fast-growing mold colonies, in addition, as a colorant, mold or yeast absorption to facilitate the observation of colonies; Chloramphenicol is an antibiotic that inhibits the growth of bacteria. AGAR as a coagulant.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
5.0 |
|
葡萄糖 Glucose |
10.0 |
|
磷酸二氢钾 Potassium dihydrogen phosphate |
1.0 |
|
硫酸镁 Magnesium sulfate |
0.5 |
|
琼脂 Agar |
15.0 |
|
孟加拉红 Bengal red |
0.033 |
|
氯霉素 chloramphenicol |
0.1 |
使用方法
Usage
称取本品31.6g，加热溶解于1000ml纯化水中，分装三角瓶，121℃高压灭菌15分钟备用。
Weigh 36.6g of this product, heat and dissolve in 1000ml purified water, divided into triangle bottles, autoclave at 121℃ for 15 minutes.
