产品名称：乳糖蛋白胨培养液
Product name：Lactose Peptone Broth
产品用途：用于饮用水，水源水中总大肠菌群的测定。
Product usage:Used for drinking water, determination of total coliform bacteria in source water.
检验原理
Test principle
蛋白胨、牛肉浸膏提供碳氮源及矿物质；乳糖是可发酵的糖类；氯化钠维持均衡的渗透压；溴甲酚紫为pH指示剂，酸性呈黄色，碱性呈紫色。
Peptone and beef extract provide carbon and nitrogen sources and minerals; Lactose is a fermentable sugar; Sodium chloride maintains a balanced osmotic pressure; Bromocresol purple is a pH indicator, yellow in acidity and purple in alkali.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 peptone |
10.0 |
|
牛肉浸膏 Beef extract |
3.0 |
|
乳糖 Lactose |
5.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
溴甲酚紫 Bromocresol purple |
0.016 |
|
PH |
7.3±0.1 |
使用方法
Usage
称取本品 2.3克，加热溶解于100ml蒸馏水中，分装到有倒立发酵管的20mm×150mm试管中，每管10ml，115℃高压灭菌20分钟备用。
Weigh 2.3 grams of this product, heat and dissolve it in 100ml distilled water, and divide it into 20mm×150mm test tubes with inverted fermentation tubes, 10ml each tube, and autoclave at 115℃ for 20 minutes.
