产品名称：卵磷脂吐温80营养琼脂
Product name：Lecithin Tween 80 Nutrient Agar
产品用途：用于化妆品细菌总数测定（GB标准）。
Product usage: Used for the determination of total bacterial count in cosmetics (GB standard).
检验原理
Test principle
蛋白胨和牛肉粉提供碳氮源、维生素和生长因子；氯化钠维持均衡的渗透压；卵磷脂和吐温80能中和防腐剂，并能起到在乳浊液中分散物质的功能，卵磷脂能中和季铵盐，吐温80 可中和酚、六氯酚、福尔马林，两者结合可中和乙醇；琼脂是培养基的凝固剂。
Peptone and beef powder provide carbon and nitrogen sources, vitamins and growth factors; Sodium chloride maintains a balanced osmotic pressure; Lecithin and Tween 80 can neutralize preservatives and disperse substances in emulsion, lecithin can neutralize quaternary ammonium salt, Tween 80 can neutralize phenol, hexachlorophenol, formalin, and the combination of both can neutralize ethanol; AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
20.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
牛肉粉 Beef powder |
3.0 |
|
卵磷脂 lecithin |
1.0 |
|
吐温80 Tween 80 |
7.0 |
|
琼脂 Agar |
15.0 |
|
PH |
7.2±0.1 25℃ |
使用方法
Usage
称取本品48.0g，加热溶解于1000ml蒸馏水中，分装，121℃高压灭菌20分钟，备用。
Weigh 48.0g of this product, heat and dissolve in 1000ml distilled water, subpack, autoclave at 121℃ for 20 minutes, set aside.
