产品名称:十六烷三甲基溴化铵琼脂
Product name:Cetrimide Agar
产品用途:用于绿脓假单胞菌的分离培养。
Product usage: Used for isolation and cultivation of Pseudomonas aeruginosa..
配方成分
Formula ingredients
明胶胰酶水解物 Gelatin trypsin hydrolysate |
20.0 |
氯化镁 Magnesium chloride |
1.4 |
硫酸钾 potassium sulphate |
10.0 |
溴化十六烷基三甲铵 Cetyltrimethylammonium bromide |
0.3 |
琼脂 Agar |
13.6 |
PH |
7.4±0.2 25℃ |
使用方法
Usage
称取本品45.3g,加入蒸馏水1000ml,添加10ml甘油,搅拌加热煮沸到完全溶解,分装,121℃高压灭菌15分钟,待冷至50℃左右,倾注无菌平皿,凝固后备用。
Weigh 45.3g of this product, add 1000ml of distilled water, add 10ml of glycerol, stir and heat to boil until completely dissolved, package, sterilize at 121 ℃ for 15 minutes, cool to around 50 ℃, pour into a sterile petri dish, solidify and set aside.