产品名称：Mueller-Hinton琼脂（MHA）
Product name：Mueller-Hinton Agar
产品用途：用于微生物药敏试验。
Product usage: For microbial susceptibility test.
检验原理：
Test principle:
牛肉粉和酸水解酪蛋白提供氮源、维生素和氨基酸；可溶性淀粉吸收有毒的代谢产物；琼脂是培养基的凝固剂。
Beef powder and acid-hydrolyzed casein provide nitrogen sources, vitamins and amino acids; Soluble starch absorbs toxic metabolites; AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
牛肉粉 Beef powder |
6.0 |
|
可溶性淀粉 Soluble starch |
1.5 |
|
酸水解酪蛋白 Acid hydrolyzed casein |
17.5 |
|
琼脂 Agar |
17.0 |
|
pH |
7.3±0.2 25℃ |
使用方法
Usage
称取本品42.0g，加人蒸馏水1000ml，搅拌加热煮沸至完全溶解，分装，121℃高压灭菌15分钟，备用。
注：培养基中含淀粉，若灭菌前未加热溶解，灭菌后可能出现少量白色沉淀。
Weigh 42.0g of this product, add 1000ml of distilled water, stir and heat to boil until completely dissolved, divided, 121 ℃ autoclave sterilization for 15 minutes, standby.
Note: The medium contains starch, if it is not heated and dissolved before sterilization, a small amount of white precipitate may appear after sterilization.
