产品名称：沙氏琼脂培养基
Product name：SDA
产品用途：用于霉菌和酵母菌的分离培养（GB/T16294-2010医药工业洁净室(区)沉降菌的测试方法）。
Product usage: Used for the isolation and culture of mold and yeast (GB/T16294-2010 pharmaceutical industry clean room (area) sedimentation bacteria test method).
检验原理
Test principle
酪蛋白胰酶消化物、 动物组织的胃酶消化物提供氮源；葡萄糖提供碳源；琼脂是培养基的凝固剂。
Peptone provides nitrogen source; Glucose provides carbon source; AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
酪蛋白胰酶消化物、动物组织的胃酶消化物 Casein trypsin digestives, gastric enzyme digestives of animal tissues |
10.0 |
|
葡萄糖 Glucose |
40.0 |
|
琼脂 Agar |
15.0 |
|
PH |
5.6±0.2 25℃ |
使用方法
Usage
称取本品65.1g，加人蒸馏水或去离子水1L，搅拌加热煮沸至完全溶解，分装三角瓶，115℃高压灭菌15分钟，备用。
Weigh 65.1g of this product, add distilled water or deionized water 1L, stirring heating and boiling until completely dissolved, divided into triangular bottles, 115 ℃ autoclave sterilization 15 minutes, standby.
