产品名称：B5培养基
Product name：B5 culture medium
产品用途：用于植物组织培养
Product usage: For plant tissue culture
配方成分
Formula ingredients
|
配方 Formula |
含量（mg/L） Content (g/L) |
|
Sodium Phosphate Monobasic/磷酸二氢钠 |
150 |
|
Calcium ChlorideAnhydrous/无水氯化钙 |
113.24 |
|
Potassium Nitrate/硝酸钾 |
2500 |
|
Ammonium Sulfate/硫酸铵 |
134 |
|
Magnesium Sulfate Anhydrous/无水硫酸镁 |
122.09 |
|
Na2EDTA/乙二胺四乙酸二钠 |
37.3 |
|
Ferrous Sulfate/硫酸亚铁 |
27.8 |
|
Manganese Sulfate/硫酸锰 |
10 |
|
Zinc Sulfate/硫酸锌 |
2 |
|
Boric Acid/硼酸 |
3 |
|
Sodium Molybdate/钼酸钠 |
0.25 |
|
Cupric Sulfate/硫酸铜 |
0.025 |
|
Cobalt Chloride.6H2O/氯化钴 |
0.025 |
|
Potassium lodide/碘化钾 |
0.75 |
|
Thiamine.HCI/盐酸硫胺素 |
10 |
|
Pyridoxine.HCI/盐酸吡哆醇 |
1 |
|
Nicotinic Acid/烟酸 |
1 |
|
Myo-Inositol/肌醇 |
100 |
|
pH值5.5 |
5.5 25℃ |
使用方法
Usage
称取本品3.21g，加热溶解于1000ml 蒸馏水中，121℃高压灭菌15分钟，备用。
注：植物组织培养基，全无机盐配方，灭菌后有少量沉淀。
Weigh 3.21g of this product, heat and dissolve it in 1000ml of distilled water, sterilize it at 121℃ for 15 minutes, and set it aside for later use.
Note: Plant tissue culture medium, formulated with complete inorganic salts, may have a small amount of sediment after sterilization.
