产品名称：1/2MS培养基（含琼脂蔗糖）
Product name：1/2 MS Medium (with Agar and Sucrose)
产品用途： 用于植物的组织培养。
Product usage: For mold and yeast culture.
配方成分
Formula ingredients
|
配方 Formula |
含量（mg/L） Content (mg/L) |
|
硝酸钾 Potassium nitrate |
950 |
|
硝酸铵 Ammonium nitrate |
825 |
|
磷酸二氢钾 Potassium dihydrogen phosphate |
85 |
|
硫酸镁 Magnesium sulfate |
185 |
|
氯化钙 Calcium chloride |
220 |
|
碘化钾 Potassium iodide |
0.83 |
|
硼酸 Boric acid |
6.2 |
|
硫酸锰 Manganese sulfate |
22.3 |
|
硫酸锌 Zinc sulfate |
8.6 |
|
钼酸钠 Sodium molybdate |
0.25 |
|
硫酸铜 Copper sulfate |
0.025 |
|
氯化钴 Cobalt chloride |
0.025 |
|
乙二胺四乙酸二钠 Disodium ethylenediaminetetraacetate |
37.3 |
|
硫酸亚铁 Ferrous sulfate |
27.8 |
|
肌醇 Inositol |
100 |
|
甘氨酸 Glycine |
2 |
|
盐酸硫胺素 Thiamine hydrochloride |
0.1 |
|
盐酸吡哆醇 Pyridoxine hydrochloride |
0.5 |
|
烟酸 Niacin |
0.5 |
|
蔗糖 Sucrose |
30000 |
|
琼脂 Agar |
7000 |
使用方法
Usage
称取本品39.45g,加热溶解于 1000ml 蒸馏水中,116℃高压灭菌 30 分钟，备用。Weigh 39.45 grams of this product, dissolve it in 1000 ml of distilled water by heating, and then subject it to high-pressure sterilization at 116°C for 30 minutes before use.
