产品名称：半固体琼脂
Product name：Semi-Solid Agar
产品用途：用于动力观察，菌种保存，H抗原位相变异试验。
Product usage: Used for dynamic observation, strain preservation, H antigen phase variation test.
检验原理
Test Principle
蛋白胨和牛肉膏粉提供氮源、维生素、矿物质；氯化钠维持均衡的渗透压；较少量的琼脂作为培养基的凝固剂。
Peptone and beef paste powder provide nitrogen sources, vitamins and minerals; Sodium chloride maintains a balanced osmotic pressure; A smaller amount of AGAR is used as a coagulant for the medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
10.0 |
|
牛肉浸粉 Beef infusion powder |
3.0 |
|
琼脂 Agar |
4.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
PH |
7.4±0.1 25℃ |
使用方法
Usage
称取本品22.0g，加热溶解于1000ml 蒸馏水中，煮沸溶解，分装小试管，121℃高压灭菌15分钟，直立凝固，备用。
Weigh 22.0g of this product, heat and dissolve it in 1000ml distilled water, boil and dissolve it, divide into small test tubes, autoclave at 121℃ for 15 minutes, set upright, set aside.
