产品名称:氯化钠结晶紫增菌液
Product name:Sodium Chloride Violet Purple Enrichment Broth
产品用途:用于副溶血性弧菌的选择性分离培养(GB标准)。
Product usage: Used for selective enhancement culture of Vibrio parahaemolyticus (GB standard).
检验原理
Test Principle
蛋白胨提供碳氮源;氯化钠维持均衡的渗透压;较高的pH抑制大肠菌群和其他杂菌的生长,有利于弧菌的生长;结晶紫对革兰氏阳性菌如葡萄球菌、 白喉杆菌,以及绿脓杆菌、白念珠菌、表皮癣菌有杀灭作用,对其他革兰氏阴性菌和抗酸菌几乎无作用。
Peptone provides carbon and nitrogen source; Sodium chloride maintains a balanced osmotic pressure; Higher pH inhibited the growth of coliform and other miscellaneous bacteria, and was conducive to the growth of Vibrio. Crystal violet has a killing effect on gram-positive bacteria such as Staphylococcus, diphtheria, as well as pseudomonas aeruginosa, candida albicans, epidermophyton, and has almost no effect on other gram-negative bacteria and acid-fast bacteria.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content (g/L) |
蛋白胨 Peptone |
20.0 |
氯化钠 Sodium chloride |
40.0 |
结晶紫 Crystal violet |
0.0005 |
PH |
9.0±0.1 25℃ |
使用方法
Usage
称取本品60.0g,加热溶解于1000ml蒸馏水中,分装,121℃高压灭菌15分钟,备用。
Weigh 60.0g of this product, heat and dissolve in 1000ml distilled water, subpack, autoclave at 121℃ for 15 minutes, set aside.