产品名称：TTC琼脂基础
Product name：TTC Agar Base
产品用途：用于弯曲杆菌的TTC试验（GB标准）。
Product usage: Used for campylobacter TTC test (GB standard).
检验原理
Test Principle
胰蛋白胨、大豆胨提供碳源、氮源和维生素、氨基酸等；氯化钠维持均衡的渗透压；葡萄糖为可发酵糖类，提供碳源；胱氨酸、硫乙醇酸钠和亚硫酸钠均有降低氧化还原电位的作用，防止过氧化物的积累对微需氧和厌氧菌的毒性。空肠弯曲菌能还原TTC，使之形成易于辨别的红色菌落；氯化血红素和维生素K1为厌氧菌提供必要的营养物质；琼脂是培养基的凝固剂。
Tryptone and soybean peptone provide carbon source, nitrogen source, vitamin, amino acid and so on. Sodium chloride maintains a balanced osmotic pressure; Glucose is fermentable carbohydrate and provides carbon source. Cystine, sodium thioglycolate and sodium sulfite can reduce the REDOX potential and prevent the accumulation of peroxide from becoming toxic to microaerobic and anaerobic bacteria. Campylobacter jejuni can reduce TTC to form a red colony that can be easily identified. Heme chloride and vitamin K1 provide essential nutrients for anaerobic bacteria; AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
胰蛋白胨 Tryptone |
17.0 |
|
大豆胨 Soybean peptone |
3.0 |
|
Nacl |
2.5 |
|
葡萄糖 Glucose |
6.0 |
|
硫乙醇酸钠 Sodium thioglycolate |
0.5 |
|
琼脂 Agar |
15.0 |
|
L-胱氨酸 L-cystine |
0.25 |
|
亚硫酸钠 Sodium sulfite |
0.1 |
|
PH |
7.2±0.1 25℃ |
使用方法
Usage
称取本品44.35克，溶解于1000ml蒸馏水中，121℃高压灭菌15min，加入无菌TTC0.4g（使用前加热煮沸30min），1%氯化血红素0.5ml，1%维生素K1 0.1ml，充分摇匀倾注无菌平板。
特别说明：1%维生素K1溶液配制方法：称取维生素K1 1g，加入无水乙醇 99ml，过滤除菌。
1%氯化血红素溶液配制方法：称取氯化血红素1g加入至少量的蒸馏水中，加入1mol/L 氢氧化钠溶液1ml溶解，然后加蒸馏水至100ml ，121℃高压灭菌15min-20min，冰箱保存。
Weigh 44.35g of this product, dissolve it in 1000ml distilled water, autoclave it at 121℃ for 15min, add 0.4g sterile TTC (heat and boil for 30min before use), 0.5ml 1% heme chloride, 0.1ml 1% vitamin K1, shake well and pour into aseptic plate.
Special note: 1% vitamin K1 solution preparation method: weigh vitamin K1 1g, add anhydrous ethanol 99ml, filter and remove bacteria.
Preparation method of 1% heme chloride solution: Weigh 1g heme chloride and add it to a small amount of distilled water, add 1mol/L sodium hydroxide solution and dissolve it in 1ml, then add distilled water to 100ml, autoclave at 121℃ for 15min-20min, and store in refrigerator.
