产品名称:艾格琼脂
Product name:Eugonic Agar
产品用途:用于生产过程中无菌检测(Acumedia方法)。
Product usage: Used for sterility testing during production (Acumedia method).
检验原理
Test principle
胰蛋白胨及大豆蛋白胨提供氮源、维生素和生长因子;葡萄糖为可发酵糖类,提供碳源;氯化钠维持平衡的渗透压;L-胱氨酸、亚硫酸钠作为还原剂,具有抗氧化作用,可保护培养基中的细菌等培养物不被氧化破坏;琼脂是凝固剂。
Tryptone and soybean peptone provide nitrogen sources, vitamins and growth factors. Glucose is fermentable carbohydrate and provides carbon source. Sodium chloride maintains equilibrium osmotic pressure; L-cystine and sodium sulfite, as reducing agents, have antioxidant effects and can protect bacteria and other cultures in the medium from being destroyed by oxidation. AGAR is a coagulant.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content (g/L) |
胰蛋白胨 Tryptone |
15.0 |
大豆蛋白胨 Soy peptone |
5.0 |
氯化钠 Sodium chloride |
4.0 |
L-cystine |
0.7 |
亚硫酸钠 Sodium sulfite |
0.2 |
葡萄糖 Glucose |
5.5 |
琼脂 Agar |
15.0 |
PH |
7.0±0.2 25℃ |
使用方法
Usage
称取本品45.4g,加热溶解于1000ml蒸馏水中,分装,121℃高压灭菌15分钟备用。
Weigh 45.4g of this product, heat and dissolve in 1000ml distilled water, dispense and autoclave at 121℃ for 15 minutes for spare.