产品名称：玫瑰红钠琼脂培养基
Product name：Rose Bengal Agar
产品用途：用于霉菌、酵母菌的计数。
Product usage: Used for counting mold and yeast.
检验原理
Test principle
蛋白胨提供碳源和氮源；葡萄糖提供能源；磷酸二氢钾为缓冲剂；硫酸镁提供必须的微量元素；玫瑰红钠作为选择性抑菌剂可抑制细菌的生长，并可减缓某些霉菌因生长过快而导致菌落漫延生长；琼脂是培养基的凝固剂。
Peptone provides carbon source and nitrogen source; Glucose provides energy; Potassium dihydrogen phosphate as buffer; Magnesium sulfate provides essential trace elements; As a selective bacteriostatic agent, rose red sodium can inhibit the growth of bacteria and slow down the spread of colonies caused by excessive growth of some molds. AGAR is a coagulant for medium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
5.0 |
|
磷酸二氢钾 Potassium dihydrogen phosphate |
1.0 |
|
玫瑰红钠 Rose red sodium |
0.0133 |
|
硫酸镁 Magnesium sulfate |
0.5 |
|
葡萄糖 Glucose |
10.0 |
|
琼脂 Agar |
14.0 |
|
PH |
6.0±0.2 25℃ |
使用方法
Usage
称取本品31.5g，加热溶解于1000ml 蒸馏水中，121℃高压灭菌15分钟备用。
Weigh 31.5g of this product, heat and dissolve in 1000ml distilled water, autoclave at 121℃ for 15 minutes and set aside.
