产品名称：龙胆紫血液琼脂基础
Product name：Crystal violet blood agar base
产品用途：用于鼠疫杆菌的选择性培养。
Product usage: Used for selective culture of S. Typhimurium.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
蛋白胨 Peptone |
10.0 |
|
牛肉粉 Beef powder |
3.0 |
|
氯化钠 Sodium chloride |
5.0 |
|
龙胆紫 Gentian violet |
0.01 |
|
琼脂 Agar |
15.0 |
|
PH |
7.0-7.2 25℃ |
使用方法
Usage
称取本品33.0g，加热溶解于1000ml蒸馏水中，121℃高压灭菌15分钟，冷至45-50℃时，加入1ml脱纤维兔血，混匀，倾入无菌平皿，备用。
Weigh 33.0g of this product, heat it and dissolve it in 1000ml distilled water, autoclave at 121℃ for 15 minutes, when it is cold to 45-50℃, add 1ml defibrillated rabbit blood, mix it well, pour it into a sterile plate and set aside.
